Among the bacteria implicated in antibiotic resistance, a group of seven pathogens identified under the term ESKAPEE bacteria constitute a serious threat. These are Gram-positive (ESKAPEE) and Gram-negative (ESKAPEE) bacteria that cause many nosocomial infections. Thus, the pyocyanin bacillus (P. aeruginosa) is for example responsible for pulmonary, urinary and post-operative infections, but also for septicemia in immunocompromised patients, suffering from cystic fibrosis or severe burn victims.
However, the prodigious adaptation capacities of these bacteria have led to the development of sophisticated resistance mechanisms to antibiotics such as the appearance of a lack of penetration, particularly through the outer membrane of Gram-negative bacteria for antibiotics presenting a periplasmic or cytoplasmic target (e.g.: reduction in membrane permeability associated with a reduced number of porins), the acquisition of efflux systems and the modification of targets...
In this context, the AGIR laboratory is therefore interested in characterizing the resistance mechanisms of clinical strains resistant to fluoroquinolones:
Then, our team also works on the development:
The synthesized molecules are then tested on a set of wild or clinical strains, included in our biobank and all presenting a fluoroquinolone resistance phenotype, but with different mechanisms.